Molecular cloning of a dehydration-responsive protein gene (MRD22) from mulberry, and determination of abiotic stress patterns of MRD22 gene expression.
Identifieur interne : 001F36 ( Main/Exploration ); précédent : 001F35; suivant : 001F37Molecular cloning of a dehydration-responsive protein gene (MRD22) from mulberry, and determination of abiotic stress patterns of MRD22 gene expression.
Auteurs : Heng Wang ; Zhaoyue Liu ; Feng Li ; Yuhua Wang ; Rongjun Fang ; Weiguo Zhao ; Long LiSource :
- Bioorganicheskaia khimiia [ 0132-3423 ]
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Analyse de séquence d'ADN (MeSH), Clonage moléculaire (MeSH), Morus (génétique), Phylogenèse (MeSH), Protéines végétales (génétique), Régulation de l'expression des gènes végétaux (MeSH), Stress physiologique (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Étiquettes de séquences exprimées (MeSH).
- MESH :
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (genetics), Expressed Sequence Tags (MeSH), Gene Expression Regulation, Plant (MeSH), Morus (genetics), Phylogeny (MeSH), Plant Proteins (genetics), Sequence Analysis, DNA (MeSH), Stress, Physiological (MeSH).
- MESH :
- chemical , genetics : DNA, Complementary, Plant Proteins.
- genetics : Morus.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Expressed Sequence Tags, Gene Expression Regulation, Plant, Phylogeny, Sequence Analysis, DNA, Stress, Physiological.
Abstract
A full-length cDNA sequence coding for Dehydration-responsive protein gene of mulberry tree, which we designated was MRD22 (GenBank accession number: JQ804833) was cloned based on mulberry expressed sequence tags (ESTs). MRD22 is 1503 bp long, contains a 334 bp 5'-UTR (untranslated region) and a 563 bp 3'-UTR, encodes 201 amino acids with a predicted molecular weight of 54.28 kDa and an isoelectric point of 9.35. Phylogenetic analysis based on MRD22 sequences from different species showed that mulberry has close relationship with Populus trichocarpa, Ricinus communis, Camellia sinensis, Gossypium hirsutum, Gossypium barbadense and so on. The expression level of the MRD22 gene under conditions of drought, low temperature and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.
PubMed: 25895340
Affiliations:
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sort="Li, Long" uniqKey="Li L" first="Long" last="Li">Long Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014 Mar-Apr</date><idno type="RBID">pubmed:25895340</idno><idno type="pmid">25895340</idno><idno type="wicri:Area/Main/Corpus">001D32</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001D32</idno><idno type="wicri:Area/Main/Curation">001D32</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001D32</idno><idno type="wicri:Area/Main/Exploration">001D32</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning of a dehydration-responsive protein gene (MRD22) from mulberry, and determination of abiotic stress patterns of MRD22 gene expression.</title><author><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name></author><author><name sortKey="Liu, Zhaoyue" sort="Liu, Zhaoyue" uniqKey="Liu Z" first="Zhaoyue" last="Liu">Zhaoyue Liu</name></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name></author><author><name sortKey="Wang, Yuhua" sort="Wang, Yuhua" uniqKey="Wang Y" first="Yuhua" last="Wang">Yuhua Wang</name></author><author><name sortKey="Fang, Rongjun" sort="Fang, Rongjun" uniqKey="Fang R" first="Rongjun" last="Fang">Rongjun Fang</name></author><author><name sortKey="Zhao, Weiguo" sort="Zhao, Weiguo" uniqKey="Zhao W" first="Weiguo" last="Zhao">Weiguo Zhao</name></author><author><name sortKey="Li, Long" sort="Li, Long" uniqKey="Li L" first="Long" last="Li">Long Li</name></author></analytic><series><title level="j">Bioorganicheskaia khimiia</title><idno type="ISSN">0132-3423</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>Expressed Sequence Tags (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Morus (genetics)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Stress, Physiological (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Morus (génétique)</term><term>Phylogenèse (MeSH)</term><term>Protéines végétales (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Étiquettes de séquences exprimées (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Morus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Morus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Expressed Sequence Tags</term><term>Gene Expression Regulation, Plant</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Phylogenèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Stress physiologique</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Étiquettes de séquences exprimées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A full-length cDNA sequence coding for Dehydration-responsive protein gene of mulberry tree, which we designated was MRD22 (GenBank accession number: JQ804833) was cloned based on mulberry expressed sequence tags (ESTs). MRD22 is 1503 bp long, contains a 334 bp 5'-UTR (untranslated region) and a 563 bp 3'-UTR, encodes 201 amino acids with a predicted molecular weight of 54.28 kDa and an isoelectric point of 9.35. Phylogenetic analysis based on MRD22 sequences from different species showed that mulberry has close relationship with Populus trichocarpa, Ricinus communis, Camellia sinensis, Gossypium hirsutum, Gossypium barbadense and so on. The expression level of the MRD22 gene under conditions of drought, low temperature and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25895340</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>03</Day></DateCompleted><DateRevised><Year>2015</Year><Month>04</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0132-3423</ISSN><JournalIssue CitedMedium="Print"><Volume>40</Volume><Issue>2</Issue><PubDate><MedlineDate>2014 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Bioorganicheskaia khimiia</Title><ISOAbbreviation>Bioorg Khim</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning of a dehydration-responsive protein gene (MRD22) from mulberry, and determination of abiotic stress patterns of MRD22 gene expression.</ArticleTitle><Pagination><MedlinePgn>203-10</MedlinePgn></Pagination><Abstract><AbstractText>A full-length cDNA sequence coding for Dehydration-responsive protein gene of mulberry tree, which we designated was MRD22 (GenBank accession number: JQ804833) was cloned based on mulberry expressed sequence tags (ESTs). MRD22 is 1503 bp long, contains a 334 bp 5'-UTR (untranslated region) and a 563 bp 3'-UTR, encodes 201 amino acids with a predicted molecular weight of 54.28 kDa and an isoelectric point of 9.35. Phylogenetic analysis based on MRD22 sequences from different species showed that mulberry has close relationship with Populus trichocarpa, Ricinus communis, Camellia sinensis, Gossypium hirsutum, Gossypium barbadense and so on. The expression level of the MRD22 gene under conditions of drought, low temperature and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Heng</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhaoyue</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Feng</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuhua</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Rongjun</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Weiguo</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Long</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Russia (Federation)</Country><MedlineTA>Bioorg Khim</MedlineTA><NlmUniqueID>7804941</NlmUniqueID><ISSNLinking>0132-3423</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031625" MajorTopicYN="N">Morus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25895340</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Fang, Rongjun" sort="Fang, Rongjun" uniqKey="Fang R" first="Rongjun" last="Fang">Rongjun Fang</name><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><name sortKey="Li, Long" sort="Li, Long" uniqKey="Li L" first="Long" last="Li">Long Li</name><name sortKey="Liu, Zhaoyue" sort="Liu, Zhaoyue" uniqKey="Liu Z" first="Zhaoyue" last="Liu">Zhaoyue Liu</name><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name><name sortKey="Wang, Yuhua" sort="Wang, Yuhua" uniqKey="Wang Y" first="Yuhua" last="Wang">Yuhua Wang</name><name sortKey="Zhao, Weiguo" sort="Zhao, Weiguo" uniqKey="Zhao W" first="Weiguo" last="Zhao">Weiguo Zhao</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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